Altered function of arcuate leptin receptor expressing neuropeptide Y neurons depending on energy balance.

ABSTRACT:

Objective

One of leptin's main targets in the hypothalamus are neuropeptide Y (NPY) neurons, with selective deletion of leptin receptors (Lepr) specifically in Npy neurons resulting in major alterations of energy partitioning between fat and bone mass. However, the specific action of these Npy+/Lepr+ neurons compared to Npy-negative Lepr (Npy-/Lepr+) neurons in regard to energy homeostasis regulation is unknown.

Methods

Specific AAV viral vectors were generated using DREADD and INTRSECT technology and used in male Lepr^Cre/+ and Lepr^Cre/+;Npy^Flp/+ mice to assess the effect of activating either all Lepr neurons or specifically Npy+/Lepr+ or Npy-/Lepr+ neurons only on feeding, energy homeostasis control, and body composition.

Results

Sele

SUBMITTER: Lee NJ 

PROVIDER: S-EPMC10460992 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature