EZH2 and matrix co-regulate phenotype and KCNB2 expression in bladder smooth muscle cells.

ABSTRACT:

Background

Partial bladder outlet obstruction (PBO) is a widespread cause of urinary dysfunction and patient discomfort, resulting in immense health care costs. Previously, we found that obstruction is associated with altered regulation of epigenetic machinery and altered function. Here we examined if PBO and chronic bladder obstructive disease (COBD) affect epigenetic marks in a proof of principle gene and explored mechanisms of its epigenetic regulation using in vitro models.

Methods

Archival obstruction tissues from COBD had been created in 200-250 g female Sprague-Dawley rats by surgical ligation of the urethra for 6 weeks, followed by removal of the suture and following animals for 6 more weeks. Obstruction (PBO) is the 6-week ligation only. Sham ligations compri

SUBMITTER: Yadav P 

PROVIDER: S-EPMC10461034 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature