Rotational Dynamics of a Protein under Shear Flow Studied by the Eckart Frame Formalism.

ABSTRACT: Proteins are natural polymers that play an essential role in both living organisms and biotechnological applications. During certain bioprocessing steps, they can be exposed to significant mechanical stress induced by, for example, shear flow or sonication, resulting in reduced therapeutic efficacy, aggregation, or even a loss of activity. For this reason, there is a need to understand and determine the susceptibility of the protein activity to the experienced mechanical stress. To acquire this knowledge, it is necessary to study the rotational dynamics of the protein. Commonly, the rotational dynamics of soft molecules is interpreted based on a theoretical analysis performed in an inertial laboratory frame. However, the obtained angular velocity mixes pure rotations and vibrations with an

SUBMITTER: Papez P 

PROVIDER: S-EPMC10461304 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature