Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart.

ABSTRACT: Optical methods for measuring intracellular ions including Ca²⁺ revolutionized our understanding of signal transduction. However, these methods are not extensively applied to intact organs due to issues including inner filter effects, motion, and available probes. Mitochondrial Ca²⁺ is postulated to regulate cell energetics and death pathways that are best studied in an intact organ. Here, we develop a method to optically measure mitochondrial Ca²⁺ and demonstrate its validity for mitochondrial Ca²⁺ and metabolism using hearts from wild-type mice and mice with germline knockout of the mitochondria calcium uniporter (MCU-KO). We previously reported that germline MCU-KO hearts do not show an impaired response to adrenergic stimulation. We find that

SUBMITTER: Kosmach A 

PROVIDER: S-EPMC10461605 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature