The Bovine Ex Vivo Retina: A Versatile Model for Retinal Neuroscience.

ABSTRACT:

Purpose

The isolated ex vivo retina is the standard model in retinal physiology and neuroscience. During isolation, the retina is peeled from the retinal pigment epithelium (RPE), which plays a key role in the visual cycle. Here we introduce the choroid-attached bovine retina as an in vivo-like model for retinal physiology. We find that-in the bovine eye-the choroid and retina can be peeled from the sclera as a single thin sheet. Importantly, the retina remains tightly associated with the RPE, which is sandwiched between the retina and the choroid. Furthermore, bovine tissue is readily available and cheap, and there are no ethical concerns related to the use of animals solely for research purposes.

Methods

We combine multi-electrode array and single-cell patch-clamp recordin

SUBMITTER: Kralik J 

PROVIDER: S-EPMC10461644 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature