Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance family.

ABSTRACT: Proteins from the Small Multidrug Resistance (SMR) family are frequently associated with horizontally transferred multidrug resistance gene arrays found in bacteria from wastewater and the human-adjacent biosphere. Recent studies suggest that a subset of SMR transporters might participate in metabolism of the common pharmaceutical metformin by bacterial consortia. Here, we show that both genomic and plasmid-associated transporters of the SMR_Gdx functional subtype export byproducts of microbial metformin metabolism, with particularly high export efficiency for guanylurea. We use solid supported membrane electrophysiology to evaluate the transport kinetics for guanylurea and native substrate guanidinium by four representative SMR_Gdx homologues. Using an internal referen

SUBMITTER: Lucero RM 

PROVIDER: S-EPMC10461911 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature