DifferentialRegulation: a Bayesian hierarchical approach to identify differentially regulated genes.

ABSTRACT:

Motivation

Although transcriptomics data is typically used to analyse mature spliced mRNA, recent attention has focused on jointly investigating spliced and unspliced (or precursor-) mRNA, which can be used to study gene regulation and changes in gene expression production. Nonetheless, most methods for spliced/unspliced inference (such as RNA velocity tools) focus on individual samples, and rarely allow comparisons between groups of samples (e.g., healthy vs. diseased). Furthermore, this kind of inference is challenging, because spliced and unspliced mRNA abundance is characterized by a high degree of quantification uncertainty, due to the prevalence of multi-mapping reads, i.e., reads compatible with multiple transcripts (or genes), and/or with both their spliced and unspl

SUBMITTER: Tiberi S 

PROVIDER: S-EPMC10462127 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature