RNA-Seq reveals ACTH-induced steroid hormone pathway participating in goat adrenal gland response to castration.

ABSTRACT: The content of androgen from adrenal is elevated under castration, and the mechanisms of compensatory secretion of adrenal androgen remain unknown. This study was designed to compare the transcript profiles between adrenals from noncastrated, orchiectomized and immunocastrated Yiling goats. Fifteen goats were randomly divided into three groups: pVAX-asd injection (control) group, pVAX-B2L-(G₄S)₃-kisspeptin-54-asd immunization (PBK-asd) group, and surgical castration (SC) group. Subsequently, serum was collected every two weeks after the initial immunization for hormone assays. At week 14 after immunization, adrenal glands were collected for transcriptome sequencing and qPCR. Serum testosterone concentration was significantly reduced in PBK-asd and SC group, demonstrat

SUBMITTER: Jing H 

PROVIDER: S-EPMC10462686 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature