Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos.

ABSTRACT: Differentiated cell nuclei can be reprogrammed after nuclear transfer (NT) to oocytes and the produced NT embryos can give rise to cloned animals. However, development of NT embryos is often hampered by recurrent reprogramming failures, including the incomplete activation of developmental genes, yet specific genes responsible for the arrest of NT embryos are not well understood. Here, we searched for developmentally important genes among the reprogramming-resistant H3K9me3-repressed genes and identified Alyref and Gabpb1 by siRNA screening. Gene knockout of Alyref and Gabpb1 by the CRISPR/Cas9 system resulted in early developmental arrest in mice. Alyref was needed for the proper formation of inner cell mass by regulating Nanog, whereas Gabpb1</

SUBMITTER: Ihashi S 

PROVIDER: S-EPMC10462978 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature