Development and validation of an effective and sensitive technique for nitrate determination in fruits and vegetables using HPLC/PDA.

ABSTRACT: This study aims to develop an effective and sensitive HPLC (High Performance Liquid Chromatography) method to determine the nitrate concentration in fruits and vegetables (F & V) using a C₁₈ column (ZORBAX Eclipse XDB-C₁₈, 80Å, 250 × 4.6 mm, 5 μm (Agilent Technologies)) maintained at 40 ⁰ C, a mobile phase made up of methanol and buffer (pentane sulfonic acid sodium salt solution), and a Photo Diode Array Detector (PDA) at 225 nm. The developed method is validated in terms of selectivity, linearity, accuracy, precision, suitability, the limit of detection (LOD), and the limit of quantification (LOQ) according to the European Union Decision 2002/657/EC. The result revealed that a ratio of 30: 70 of the organic modifier methanol and buffer with pH 2.8 shows t

SUBMITTER: Uddin R 

PROVIDER: S-EPMC10463336 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature