RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter.

ABSTRACT:

Background

Megakaryocytes (MKs) are platelet precursors, which arise from hematopoietic stem cells (HSCs). While MK lineage commitment and differentiation are accompanied by changes in gene expression, many factors that modulate megakaryopoiesis remain to be uncovered. Replication initiation determinant protein (RepID) which has multiple histone-code reader including bromodomain, cryptic Tudor domain and WD40 domains and Cullin 4-RING E3 ubiquitin ligase complex (CRL4) recruited to chromatin mediated by RepID have potential roles in gene expression changes via epigenetic regulations. We aimed to investigate whether RepID-CRL4 participates in transcriptional changes required for MK differentiation.

Methods

The PCR array was performed using cDNAs derived from RepID-proficient

SUBMITTER: Jo JH 

PROVIDER: S-EPMC10463337 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature