Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway.

ABSTRACT:

Background

To elucidate the role of Mucin1 (MUC1) in the trophoblast function (glucose uptake and apoptosis) of gestational diabetes mellitus (GDM) women through the Wnt/β-catenin pathway.

Methods

Glucose uptake was analyzed by plasma GLUT1 and GLUT4 levels with ELISA and measured by the expression of GLUT4 and INSR with immunofluorescence and Western blotting. Apoptosis was measured by the expression of Bcl-2 and Caspase3 by Western blotting and flow cytometry. Wnt/β-catenin signaling measured by Western blotting. In vitro studies were performed using HTR-8/SVneo cells that were cultured and treated with high glucose (HG), sh-MUC1 and FH535 (inhibitor of Wnt/β-catenin signaling).

Results

MUC1 was highly expressed in the placental trophoblasts of GDM, and the Wnt/β-ca

SUBMITTER: Cui SS 

PROVIDER: S-EPMC10463356 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature