MicroRNA-338-3p helps regulate ovarian function by affecting granulosa cell function and early follicular development.

ABSTRACT:

Background

Follicular development in mammalian ovaries is a complex and dynamic process, and the interactions and regulatory-feedback loop between the follicular microenvironment, granulosa cells (GCs), and oocytes can affect follicular development and normal ovary functions. Abnormalities in any part of the process may cause abnormal follicular development, resulting in infertility. Hence, exploring the pathogenesis of abnormal follicular development is extremely important for diagnosing and treating infertile women.

Methods

RNA sequencing was performed with ovarian cortical tissues established in vitro. In situ-hybridization assays were performed to study microRNA-338-3p (miR-338-3p) expressed in GCs and oocytes. In vitro culture models were established with GCs and neonat

SUBMITTER: Xu Z 

PROVIDER: S-EPMC10463366 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature