Whole-cell catalyze L-dopa to dopamine via co-expression of transport protein AroP in Escherichia coli.

ABSTRACT: Dopamine is high-value compound of pharmaceutical interest, but its industrial scale production mostly focuses on chemical synthesis, possessing environment pollution. Bio-manufacturing has caused much attention for its environmental characteristic. Resting cells were employed to as biocatalysts with extraordinary advantages like offering stable surroundings, the inherent presence of expensive cofactors. In this study, whole-cell bioconversion was employed to convert dopa to dopamine. To increase the titer and yield of dopamine production through whole-cell catalysis, three kinds of aromatic amino acid transport protein, AroP, PheP and TyrP, were selected to be co-expressed. The effects of the concentration of L-dopa, pyridoxal-5'- phosphate (PLP), reaction temperature and pH were characte

SUBMITTER: Gao S 

PROVIDER: S-EPMC10463401 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature