GREM1 is a potential biomarker for the progression and prognosis of bladder cancer.

ABSTRACT:

Background

Gremlin-1 (GREM1) is a protein closely related to tumor growth, although its function in bladder cancer (BCa) is currently unknown. Our first objective was to study the GREM1 treatment potential in BCa.

Methods

BCa tissue samples were collected for the detection of GREM1 expression using Western blot analysis and Immunofluorescence staining. Association of GREM1 expression with clinicopathology and prognosis as detected by TCGA (The Cancer Genome Atlas) database. The functional investigation was tested by qRT-PCR, western blot analysis, CCK-8, cell apoptosis, wound healing, and transwell assays. The interaction between GREM1 and the downstream PI3K/AKT signaling pathway was assessed by Western blot analysis.

Results

GREM1 exhibited high expression in BCa ti

SUBMITTER: Jiang PC 

PROVIDER: S-EPMC10463405 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature