Evaluation of the pooled sample method in Infinium MethylationEPIC BeadChip array by comparison with individual samples.

ABSTRACT:

Background

The pooled sample method is used in epigenomic research and expression analysis and is a cost-effective screening approach for small amounts of DNA. Evaluation of the pooled sample method in epigenomic studies is performed using the Illumina Infinium Methylation 450K BeadChip array; however, subsequent reports on the updated 850K array are lacking. A previous study demonstrated that the methylation levels obtained from individual samples were accurately replicated using pooled samples but did not address epigenome-wide association study (EWAS) statistics. The DNA quantification method, which is important for the homogeneous mixing of DNA in the pooled sample method, has since become fluorescence-based, and additional factors need to be considered including the resolution

SUBMITTER: Nishitani S 

PROVIDER: S-EPMC10463626 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature