A streamlined guide RNA screening system for genome editing in Sorghum bicolor.

ABSTRACT:

Background

Genome editing tools derived from clustered regularly interspaced short palindromic repeats (CRISPR) systems have been developed for generating targeted mutations in plants. Although these tools hold promise for rapid crop improvement, target-specific guide RNAs exhibit variable activity. To improve genome editing, a rapid and precise method for evaluating their efficiency is necessary.

Results

Here we report an efficient system for screening single guide RNAs (sgRNAs) for genome editing in sorghum using a transient protoplast transfection assay. Protoplasts were isolated from leaves from sorghum plants cultivated under three different conditions. Cultivation for three days of continuous darkness following seven days with a 16-h light and 8-h dark photoperiod resu

SUBMITTER: Lee JS 

PROVIDER: S-EPMC10463630 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature