A high-resolution melting approach for the simultaneous differentiation of five human babesiosis-causing Babesia species.

ABSTRACT:

Background

Six species of apicomplexan parasites of the genus Babesia, namely B. microti, B. divergens, B. duncani, B. motasi, B. crassa-like and B. venatorum, are considered to be the primary causal agents of human babesiosis in endemic areas. These six species possess variable degrees of virulence for their primary hosts. Therefore, the accurate identification of these species is critical for the adoption of appropriate therapeutic strategies.

Methods

We developed a real-time PCR-high-resolution melting (qPCR-HRM) approach targeting 18S ribosomal RNA gene of five Babesia spp. based on melting temperature (T_m) and genotype confidence percentage values. This approach was then evaluated using 429 blood samples collected from patients with a history of tick bites, 1

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10463647 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature