The impact of storage buffer and storage conditions on fecal samples for bacteriophage infectivity and metavirome analyses.

ABSTRACT:

Background

There is an increasing interest in investigating the human gut virome for its influence on the gut bacterial community and its putative influence on the trajectory towards health or disease. Most gut virome studies are based on sequencing of stored fecal samples. However, relatively little is known about how conventional storage buffers and storage conditions affect the infectivity of bacteriophages and influence the downstream metavirome sequencing.

Results

We demonstrate that the infectivity and genome recovery rate of different spiked bacteriophages (T4, c2 and Phi X174) are variable and highly dependent on storage buffers. Regardless of the storage temperature and timespan, all tested phages immediately lost 100% (DNA/RNA Shield) or more than 90% (StayRNA and

SUBMITTER: Zhai X 

PROVIDER: S-EPMC10463696 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature