Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases.

ABSTRACT: A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.

SUBMITTER: Vahaboglu H 

PROVIDER: S-EPMC104637 | biostudies-literature | 1998 Mar

REPOSITORIES: biostudies-literature