Successful use of HTF as a basal fertilization medium during SEcuRe mouse in vitro fertilization.

ABSTRACT:

Objective

The ever-increasing number of genetically engineered mouse models highlights the need for efficient archiving and distribution of these lines. Sperm cryopreservation has become the preferred technique for the majority of these models due to its low requirement of costs, time, and experimental animals. Yet, current in vitro fertilization (IVF) protocols either exhibit decreased fertilization efficiency for the most popular C57BL/6 strain, as recently demonstrated by us, or require costly and difficult-to-prepare media, respectively. As a result, we previously developed SEcuRe, a modified IVF protocol with low costs and high fertilization efficiency. The popular basal fertilization medium, Cook's^® proprietary "Research vitro fert" (RVF), used in this protocol has

SUBMITTER: Wigger M 

PROVIDER: S-EPMC10463834 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature