Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest.

ABSTRACT:

Background

For decoding the mechanism of how cells and organs function information on their ultrastructure is essential. High-resolution 3D imaging has revolutionized morphology. Serial block face scanning electron microscopy (SBF-SEM) offers non-laborious, automated imaging in 3D of up to ~ 1 mm³ large biological objects at nanometer-scale resolution. For many samples there are obstacles. Quality imaging is often hampered by charging effects, which originate in the nonconductive resin used for embedding. Especially, if the imaged region of interest (ROI) includes the surface of the sample and neighbours the empty resin, which insulates the object. This extra resin also obscures the sample's morphology, thus making navigation to the ROI difficult.

Results

Using th

SUBMITTER: Konopova B 

PROVIDER: S-EPMC10463905 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature