Highly multiplexed targeted sequencing strategy for infectious disease surveillance.

ABSTRACT:

Background

Global efforts to characterize diseases of poverty are hampered by lack of affordable and comprehensive detection platforms, resulting in suboptimal allocation of health care resources and inefficient disease control. Next generation sequencing (NGS) can provide accurate data and high throughput. However, shotgun and metagenome-based NGS approaches are limited by low concentrations of microbial DNA in clinical samples, requirements for tailored sample and library preparations plus extensive bioinformatics analysis. Here, we adapted molecular inversion probes (MIPs) as a cost-effective target enrichment approach to characterize microbial infections from blood samples using short-read sequencing. We designed a probe panel targeting 2 bacterial genera, 21 bacterial and 6 fu

SUBMITTER: Hernandez-Neuta I 

PROVIDER: S-EPMC10463907 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature