Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors.

ABSTRACT:

Background

The interface zone, area around invasive carcinoma, can be thought of as the actual tissue of the tumor microenvironment with precedent alterations for tumor invasion. However, the heterogeneity and characteristics of the microenvironment in the interface area have not yet been thoroughly explored.

Methods

For in vitro studies, single-cell RNA sequencing (scRNA-seq) was used to characterize the cells from the tumor zone, the normal zone and the interface zone with 5-mm-wide belts between the tumor invasion front and the normal zone. Through scRNA-seq data analysis, we compared the cell types and their transcriptional characteristics in the different zones. Pseudotime, cell-cell communication and pathway analysis were performed to characterize the zone-specific mic

SUBMITTER: Yang W 

PROVIDER: S-EPMC10463980 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature