The RNA m6A modification might participate in microglial activation during hypoxic-ischemic brain damage in neonatal mice.

ABSTRACT:

Background

The RNA m6A modification has been implicated in multiple neurological diseases as well as macrophage activation. However, whether it regulates microglial activation during hypoxic-ischemic brain damage (HIBD) in neonates remains unknown. Here, we aim to examine whether the m6A modification is involved in modulating microglial activation during HIBD. We employed an oxygen and glucose deprivation microglial model for in vitro studies and a neonatal mouse model of HIBD. The brain tissue was subjected to RNA-seq to screen for significant changes in the mRNA m6A regulator. Thereafter, we performed validation and bioinformatics analysis of the major m6A regulators.

Results

RNA-seq analysis revealed that, among 141 m6A regulators, 31 exhibited significant differential ex

SUBMITTER: Su X 

PROVIDER: S-EPMC10463984 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature