RNF173 suppresses RAF/MEK/ERK signaling to regulate invasion and metastasis via GRB2 ubiquitination in Hepatocellular Carcinoma.

ABSTRACT:

Background

The role of the membrane-associated RING-CH (MARCH) family in carcinogenesis has been widely studied, but the member of this family, RNF173, has not yet been thoroughly explored in the context of hepatocellular carcinoma (HCC).

Methods

With the use of an HCC tissue microarray and IHC staining, we aim to determine the differential expression of RNF173 in HCC patients and its clinical significance. The biological role of RNF173 is investigated through in vitro and in vivo experiments. RNA sequencing, mass spectrometry, and immunoprecipitation are performed to uncover the underlying mechanism of RNF173's impact on the development of HCC.

Results

The mRNA and protein levels of RNF173 were significantly lower in HCC tissues than in normal tissues. HCC patients w

SUBMITTER: Zhou J 

PROVIDER: S-EPMC10464048 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature