Long-read assembled metagenomic approaches improve our understanding on metabolic potentials of microbial community in mangrove sediments.

ABSTRACT:

Background

Mangrove wetlands are coastal ecosystems with important ecological features and provide habitats for diverse microorganisms with key roles in nutrient and biogeochemical cycling. However, the overall metabolic potentials and ecological roles of microbial community in mangrove sediment are remained unanswered. In current study, the microbial and metabolic profiles of prokaryotic and fungal communities in mangrove sediments were investigated using metagenomic analysis based on PacBio single-molecule real time (SMRT) and Illumina sequencing techniques.

Results

Comparing to Illumina short reads, the incorporation of PacBio long reads significantly contributed to more contiguous assemblies, yielded more than doubled high-quality metagenome-assembled genomes (MAGs), and

SUBMITTER: Zhang ZF 

PROVIDER: S-EPMC10464287 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature