Investigating phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol.

ABSTRACT:

Background

Chromatin-associated phase separation proteins establish various biomolecular condensates via liquid-liquid phase separation (LLPS), which regulates vital biological processes spatially and temporally. However, the widely used methods to characterize phase separation proteins are still based on low-throughput experiments, which consume time and could not be used to explore protein LLPS properties in bulk.

Results

By combining gradient 1,6-hexanediol (1,6-HD) elution and quantitative proteomics, we developed chromatin enriching hexanediol separation coupled with liquid chromatography-mass spectrometry (CHS-MS) to explore the LLPS properties of different chromatin-associated proteins (CAPs). First, we found that CAPs were enriched more effectively in the 1,6-HD trea

SUBMITTER: Zhu P 

PROVIDER: S-EPMC10464338 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature