Peripheral immune landscape for hypercytokinemia in myasthenic crisis utilizing single-cell transcriptomics.

ABSTRACT:

Background

Myasthenia gravis (MG) is the most prevalent autoimmune disorder affecting the neuromuscular junction. A rapid deterioration in respiratory muscle can lead to a myasthenic crisis (MC), which represents a life-threatening condition with high mortality in MG. Multiple CD4⁺ T subsets and hypercytokinemia have been identified in the peripheral pro-inflammatory milieu during the crisis. However, the pathogenesis is complicated due to the many types of cells involved, leaving the underlying mechanism largely unexplored.

Methods

We conducted single-cell transcriptomic and immune repertoire sequencing on 33,577 peripheral blood mononuclear cells (PBMCs) from two acetylcholine receptor antibody-positive (AChR +) MG patients during MC and again three months post-

SUBMITTER: Zhong H 

PROVIDER: S-EPMC10464341 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature