Tuning transcription factor DegU for developing extracellular protease overproducer in Bacillus pumilus.

ABSTRACT:

Background

Global transcription machinery engineering (gTME) is an effective approach employed in strain engineering to rewire gene expression and reshape cellular metabolic fluxes at the transcriptional level.

Results

In this study, we utilized gTME to engineer the positive transcription factor, DegU, in the regulation network of major alkaline protease, AprE, in Bacillus pumilus. To validate its functionality when incorporated into the chromosome, we performed several experiments. First, three negative transcription factors, SinR, Hpr, and AbrB, were deleted to promote AprE synthesis. Second, several hyper-active DegU mutants, designated as DegU(hy), were selected using the fluorescence colorimetric method with the host of the Bacillus subtilis ΔdegSU mutant. Third, we int

SUBMITTER: Xie CY 

PROVIDER: S-EPMC10464342 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature