The circadian clock circuitry modulates leukemia initiating cell activity in T-cell acute lymphoblastic leukemia.

ABSTRACT:

Background

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy, characterized by restricted cellular subsets with asymmetrically enriched leukemia initiating cell (LIC) activity. Nonetheless, it is still unclear which signaling programs promote LIC maintenance and progression.

Methods

Here, we evaluated the role of the biological clock in the regulation of the molecular mechanisms and signaling pathways impacting the cellular dynamics in T-ALL through an integrated experimental approach including gene expression profiling of shRNA-modified T-ALL cell lines and Chromatin Immunoprecipitation Sequencing (ChIP-Seq) of leukemic cells. Patient-derived xenograft (PDXs) cell subsets were also genetically manipulated in order to assess the LIC activi

SUBMITTER: Murgo E 

PROVIDER: S-EPMC10464343 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature