Duplex loop-mediated isothermal amplification assay for simultaneous detection of human and human male DNA.

ABSTRACT:

Objective

Screening of human and human male DNA is necessary for forensic DNA analyses. Although quantitative real-time PCR (qPCR) is commonly used for detecting and quantifying these DNA targets, its use as a screening tool is time-consuming and labor-intensive. To streamline and simplify the screening process, we aimed to develop a duplex loop-mediated isothermal amplification (LAMP) assay capable of simultaneously detecting human and human male DNA in a single tube. We assessed the duplex LAMP assay for forensic application.

Results

For our duplex LAMP assay, we have utilized two fluorescent probes with HEX and FAM fluorophores to specifically detect human and human male DNA, respectively. The HEX (human target) signal was detected from both the male and female DNA sample

SUBMITTER: Kubo S 

PROVIDER: S-EPMC10464483 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature