The effect of mycophenolate mofetil on podocytes in nephrotoxic serum nephritis.

ABSTRACT: Mycophenolate mofetil (MMF) is applied in proteinuric kidney diseases, but the exact mechanism of its effect on podocytes is still unknown. Our previous in vitro experiments suggested that MMF can ameliorate podocyte damage via restoration of the Ca²⁺-actin cytoskeleton axis. The goal of this study was to characterize podocyte biology during MMF treatment in nephrotoxic serum (NTS) nephritis (NTN). NTN was induced in three-week old wild-type mice. On day 3, half of the mice were treated with MMF (100 mg/kgBW/d p.o.) for one week. On day 10, we performed proteomic analysis of glomeruli as well as super-resolution imaging of the slit diaphragm. For multiphoton imaging of Ca²⁺ concentration ([Ca²⁺]_i), the experimental design was repeated in mice exp

SUBMITTER: Hackl A 

PROVIDER: S-EPMC10465485 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature