2-Photon imaging of fluorescent proteins in living swine.

ABSTRACT: A common point of failure in translation of preclinical neurological research to successful clinical trials comes in the giant leap from rodent models to humans. Non-human primates are phylogenetically close to humans, but cost and ethical considerations prohibit their widespread usage in preclinical trials. Swine have large, gyrencencephalic brains, which are biofidelic to human brains. Their classification as livestock makes them a readily accessible model organism. However, their size has precluded experiments involving intravital imaging with cellular resolution. Here, we present a suite of techniques and tools for in vivo imaging of porcine brains with subcellular resolution. Specifically, we describe surgical techniques for implanting a synthetic, flexible, transparent dural window f

SUBMITTER: Costine-Bartell BA 

PROVIDER: S-EPMC10465491 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature