Targeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development.

ABSTRACT: Tissues take shape through a series of morphogenetic movements guided by local cell-scale mechanical forces. Current in vitro approaches to recapitulate tissue mechanics rely on uncontrolled self-organization or on the imposition of extrinsic and homogenous forces using matrix or instrument-driven stimulation, thereby failing to recapitulate highly localized and spatially varying forces. Here we develop a method for targeted mechanical stimulation of organoids using embedded magnetic nanoparticles. We show that magnetic clusters within organoids can be produced by sequential aggregation of magnetically labeled and non-labeled human pluripotent stem cells. These clusters impose local mechanical forces on the surrounding cells in response to applied magnetic fields. We show that precise, spa

SUBMITTER: Abdel Fattah AR 

PROVIDER: S-EPMC10465512 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature