Importance of residue 248 in Escherichia coli RNase P RNA mediated cleavage.

ABSTRACT: tRNA genes are transcribed as precursors and RNase P generates the matured 5' end of tRNAs. It has been suggested that residue - 1 (the residue immediately 5' of the scissile bond) in the pre-tRNA interacts with the well-conserved bacterial RNase P RNA (RPR) residue A₂₄₈ (Escherichia coli numbering). The way A₂₄₈ interacts with residue - 1 is not clear. To gain insight into the role of A₂₄₈, we analyzed cleavage as a function of A₂₄₈ substitutions and N_-1 nucleobase identity by using pre-tRNA and three model substrates. Our findings are consistent with a model where the structural topology of the active site varies and depends on the identity of the nucleobases at, and in proximity to, the cleavage site and their potential to interact.

SUBMITTER: Mao G 

PROVIDER: S-EPMC10465520 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature