Dimerization of iLID optogenetic proteins observed using 3D single-molecule tracking in live E. coli.

ABSTRACT: 3D single-molecule tracking microscopy has enabled measurements of protein diffusion in living cells, offering information about protein dynamics and cellular environments. For example, different diffusive states can be resolved and assigned to protein complexes of different size and composition. However, substantial statistical power and biological validation, often through genetic deletion of binding partners, are required to support diffusive state assignments. When investigating cellular processes, real-time perturbations to protein spatial distributions is preferable to permanent genetic deletion of an essential protein. For example, optogenetic dimerization systems can be used to manipulate protein spatial distributions that could offer a means to deplete specific diffusive states ob

SUBMITTER: Achimovich AM 

PROVIDER: S-EPMC10465707 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature