Air-ventilated normothermic machine perfusion alleviates hepatic injury from DCD rat through CYP1A2.

ABSTRACT:

Background

Normothermic machine perfusion (NMP) could provide protection to organs from donation after circulatory death (DCD) before transplantation, and its molecular mechanism remains unclear. Our previous study discovered that the air-ventilated NMP confers a better DCD liver recovery than oxygen-ventilated NMP. The purpose in the current study was to investigate the protective mechanism of air-ventilated NMP in a rat model of DCD liver by metabolomics, and to select biomarker to predict liver function recovery.

Materials and methods

Peroxisome proliferator activator receptor-α (PPARα) agonist or antagonist was administered via the perfusion circuit in the air-ventilated NMP. Perfusate samples were taken for measurements of aminotransferases using standard biochem

SUBMITTER: Shi JH 

PROVIDER: S-EPMC10465863 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature