The Inositol Pyrophosphate Biosynthetic Pathway of Trypanosoma cruzi.

ABSTRACT: Inositol phosphates (IPs) are phosphorylated derivatives of myo-inositol involved in the regulation of several cellular processes through their interaction with specific proteins. Their synthesis relies on the activity of specific kinases that use ATP as phosphate donor. Here, we combined reverse genetics and liquid chromatography coupled to mass spectrometry (LC-MS) to dissect the inositol phosphate biosynthetic pathway and its metabolic intermediates in the main life cycle stages (epimastigotes, cell-derived trypomastigotes, and amastigotes) of Trypanosoma cruzi, the etiologic agent of Chagas disease. We found evidence of the presence of highly phosphorylated IPs, like inositol hexakisphosphate (IP₆), inositol heptakisphosphate (IP₇), and inositol octakisphos

SUBMITTER: Mantilla BS 

PROVIDER: S-EPMC10466500 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature