Metabolic engineering strategies for naringenin production enhancement in Streptomyces albidoflavus J1074.

ABSTRACT:

Background

Naringenin is an industrially relevant compound due to its multiple pharmaceutical properties as well as its central role in flavonoid biosynthesis.

Results

On our way to develop Streptomyces albidoflavus J1074 as a microbial cell factory for naringenin production, we have significantly increased the yields of this flavanone by combining various metabolic engineering strategies, fermentation strategies and genome editing approaches in a stepwise manner. Specifically, we have screened different cultivation media to identify the optimal production conditions and have investigated how the additive feeding of naringenin precursors influences the production. Furthermore, we have employed genome editing strategies to remove biosynthetic gene clusters (BGCs) associated w

SUBMITTER: Ye S 

PROVIDER: S-EPMC10466684 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature