Okadaic Acid Detection through a Rapid and Sensitive Amplified Luminescent Proximity Homogeneous Assay.

ABSTRACT: Okadaic acid (OA), a marine biotoxin produced by microalgae, poses a significant threat to mariculture, seafood safety, and human health. The establishment of a novel, highly sensitive detection method for OA would have significant practical and scientific implications. Therefore, the purpose of this study was to develop an innovative approach for OA detection. A competitive amplified luminescent proximity homogeneous assay (AlphaLISA) was developed using the principle of specific antigen-antibody binding based on the energy transfer between chemiluminescent microspheres. The method was non-washable, sensitive, and rapid, which could detect 2 × 10^-2-200 ng/mL of OA within 15 min, and the detection limit was 4.55 × 10^-3 ng/mL. The average intra- and inter-assay coeffic

SUBMITTER: Qin Y 

PROVIDER: S-EPMC10467062 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature