Dataset Information

Microbially Induced Calcium Carbonate Precipitation by Sporosarcina pasteurii: a Case Study in Optimizing Biological CaCO₃ Precipitation.

ABSTRACT: Current production of traditional concrete requires enormous energy investment that accounts for approximately 5 to 8% of the world's annual CO₂ production. Biocement is a building material that is already in industrial use and has the potential to rival traditional concrete as a more convenient and more environmentally friendly alternative. Biocement relies on biological structures (enzymes, cells, and/or cellular superstructures) to mineralize and bind particles in aggregate materials (e.g., sand and soil particles). Sporosarcina pasteurii is a workhorse organism for biocementation, but most research to date has focused on S. pasteurii as a building material rather than a biological system. In this review, we synthesize available materials science, microbiology, biochemistry, and cell biology evidence regarding biological CaCO₃ precipitation and the role of microbes in microbially induced calcium carbonate precipitation (MICP) with a focus on S. pasteurii. Based on the available information, we provide a model that describes the molecular and cellular processes involved in converting feedstock material (urea and Ca²⁺) into cement. The model provides a foundational framework that we use to highlight particular targets for researchers as they proceed into optimizing the biology of MICP for biocement production.

SUBMITTER: Carter MS

PROVIDER: S-EPMC10467343 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Publications

Microbially Induced Calcium Carbonate Precipitation by Sporosarcina pasteurii: a Case Study in Optimizing Biological CaCO3 Precipitation.

Carter Michael S MS Tuttle Matthew J MJ Mancini Joshua A JA Martineau Rhett R Hung Chia-Suei CS Gupta Maneesh K MK

Applied and environmental microbiology 20230713 8

Current production of traditional concrete requires enormous energy investment that accounts for approximately 5 to 8% of the world's annual CO2 production. Biocement is a building material that is already in industrial use and has the potential to rival traditional concrete as a more convenient and more environmentally friendly alternative. Biocement relies on biological structures (enzymes, cells, and/or cellular superstructures) to mineralize and bind particles in aggregate materia ...[more]

PMID: 37439668

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Project description:Simple plugging of the high-permeability "thief zones" of oil reservoirs is the most plausible and also the most straightforwardly achievable approach to enhance sweep efficiency and oil recovery. Sporosarcina pasteurii is a representative microorganism with the ability to precipitate calcium carbonate (CaCO3) via enzymatic hydrolysis of urea in the presence of calcium ions. Microbially induced calcium carbonate precipitation (MICP) can cement and seal the granular and fractured media and thus can be used as a potential microbial plugging agent for the high-permeability zones of oil reservoirs. The following investigated the microscopic characteristics of MICP plugging and its efficacy in permeability reduction. The columns of near-spherical silica sand and angular silica sand with three separate granularities (40/60, 60/80, and 80/120 mesh) were used as artificial rock cores representing distinct pore sizes and pore characteristics to investigate the efficacy and microprocess of MICP plugging with different biotreatment periods. The results indicated that permeability is reduced significantly after only short periods of biotreatment. After eight cycles of MICP treatments, the permeability for each type of cores dropped by 54-90% of individual initial permeabilities. The measured CaCO3 content indicated that the decreasing rate in permeability with the increasing CaCO3 content experiences three contrasting stages, namely, slow decline, speedy decline, and plateauing. X-ray diffraction indicated that most of the generated CaCO3 crystals occur as vaterite with only a small amount of calcite. Imaging by scanning electron microscopy further revealed the microprocess of MICP plugging. Microorganisms first concentrate on the pore wall to secrete CaCO3, forming a thin and large uniform layer of CaCO3. Then, some nucleation sites of CaCO3 crystals will experience further preferential growth, resulting in large, dominant crystals that act as a plugging agent within the pore space. Compared to extracellular polymeric substances, which are currently the primary microbial plugging agent used to enhance sweep efficiency of oil reservoirs, bio-CaCO3 appears more effective in plugging in terms of its morphology, size, and growth characteristics.

Dataset Information

Microbially Induced Calcium Carbonate Precipitation by Sporosarcina pasteurii: a Case Study in Optimizing Biological CaCO₃ Precipitation.

Publications

Microbially Induced Calcium Carbonate Precipitation by <i>Sporosarcina pasteurii</i>: a Case Study in Optimizing Biological CaCO<sub>3</sub> Precipitation.

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