Discovery of cisplatin-binding proteins by competitive cysteinome profiling.

ABSTRACT: Cisplatin is a widely used cancer metallodrug that induces cytotoxicity by targeting DNA and chelating cysteines in proteins. Here we applied a competitive activity-based protein profiling strategy to identify cisplatin-binding cysteines in cancer proteomes. A novel cisplatin target, MetAP1, was identified and functionally validated to contribute to cisplatin's cytotoxicity.

SUBMITTER: Wang X 

PROVIDER: S-EPMC10467758 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature