Small extrachromosomal circular DNAs as biomarkers for multi-cancer diagnosis and monitoring.

ABSTRACT:

Background

Small extrachromosomal circular DNAs (eccDNAs) have the potential to be cancer biomarkers. However, the formation mechanisms and functions of small eccDNAs selected in carcinogenesis are not clear, and whether the small eccDNA profile in the plasma of cancer patients represents that in cancer tissues remains to be elucidated.

Methods

A novel sequencing workflow based on the nanopore sequencing platform was used to sequence naturally existing full-length small eccDNAs in tissues and plasma collected from 25 cancer patients (including prostate cancer, hepatocellular carcinoma and colorectal cancer), and from an independent validation cohort (including 7 cancer plasma and 14 healthy plasma).

Results

Compared with those in non-cancer tissues, small eccDNAs dete

SUBMITTER: Luo X 

PROVIDER: S-EPMC10468585 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature