The coenzyme B₁₂ precursor 5,6-dimethylbenzimidazole is a flavin antagonist in Salmonella.

ABSTRACT: Salmonella enterica subsp. enterica sv. Typhimurium str. LT2 (hereafter S. Typhimurium) synthesizes adenosylcobalamin (AdoCbl, CoB₁₂) de novo only under anoxic conditions, but it can assemble the lower ligand loop (a.k.a. the nucleotide loop) and can form the unique C-Co bond present in CoB₁₂ in the presence or absence of molecular oxygen. During studies of nucleotide loop assembly in S. Typhimurium, we noticed that the growth of this bacterium could be arrested by the lower ligand nucleobase, namely 5,6-dimethylbenzimidazole (DMB). Here we report in vitro and in vivo evidence that shows that the structural similarity of DMB to the isoalloxazine moiety of flavin cofactors causes its deleterious effect on cell growth. We

SUBMITTER: Malalasekara L 

PROVIDER: S-EPMC10468695 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature