Single-cell sequencing reveals the existence of fetal vascular endothelial stem cell-like cells in mouse liver.

ABSTRACT:

Background

A resident vascular endothelial stem cell (VESC) population expressing CD157 and CD200 has been identified recently in the adult mouse. However, the origin of this population and how it develops has not been characterized, nor has it been determined whether VESC-like cells are present during the perinatal period. Here, we investigated the presence of perinatal VESC-like cells and their relationship with the adult VESC-like cell population.

Methods

We applied single-cell RNA sequencing of endothelial cells (ECs) from embryonic day (E) 14, E18, postnatal day (P) 7, P14, and week (W) 8 liver and investigated transcriptomic changes during liver EC development. We performed flow cytometry, immunofluorescence, colony formation assays, and transplantation assays to valid

SUBMITTER: Rahmawati FN 

PROVIDER: S-EPMC10468894 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature