B cell receptors and free antibodies have different antigen-binding kinetics.

ABSTRACT: Since the pioneering works of Berg and Purcell, discriminating between diffusion followed by binding has played a central role in understanding cell signaling. B cell receptors (BCR) and antibodies (Ab) challenge that simplified view as binding to the antigen follows after a chain of diffusion and rotations, including whole molecule rotation and independent tilts and twists of their Fab arms due to their Y-shaped structure and flexibility. In this paper, we combine analytical calculations with Brownian simulations to derive the first-passage times due to these three rotations positioning the Fab paratopes at a proper distance and orientation required for antigen binding. Our results indicate that when measuring Ab-Ag effective kinetic binding rates, using experimental methods in which the

SUBMITTER: Garcia-Sanchez M 

PROVIDER: S-EPMC10469035 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature