Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics.

ABSTRACT: Dermal papilla cells (DPCs) play roles in key functions of the epidermis such as hair generation. The use of human induced pluripotent cells (hiPSCs) makes it possible to obtain DP-like cells and study the molecular mechanisms of DPC development during embryogenesis. In this work, we studied the phenotypic trajectory of hiPSCs during their differentiation into DP-like cells and evaluated the epithelial-mesenchymal interaction potential of the resulting cell line. Specifically, we differentiated hiPSCs into neural progenitor cells (NPCs) and subsequently into DP-like cells. Analysis of bulk RNA-seq data during this process enabled us to observe gene expression dynamics during five stages of dermal differentiation. Furthermore, functional assays (organoids in both collagen gels and hanging d

SUBMITTER: Riabinin A 

PROVIDER: S-EPMC10469169 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature