The utilization of nanopore targeted sequencing proves to be advantageous in the identification of infections present in deceased donors.

ABSTRACT:

Background

Nanopore Target Sequencing (NTS) represents a novel iteration of gene sequencing technology; however, its potential utility in the detection of infection in deceased donors has yet to be documented. The present study endeavors to assess the applicability of NTS in this domain.

Methods

This retrospective study comprised a cohort of 71 patients who were under intensive care at Renmin Hospital of Wuhan University between June 2020 and January 2022. The specimens were subjected to microbiological tests utilizing NTS, culture, and other techniques, and subsequently, the diagnostic accuracy of NTS was compared with conventional methods.

Results

Blood NTS exhibited a better agreement rate of 52.11% and a greater positive rate of pathogen detection than blood cultu

SUBMITTER: Yao Z 

PROVIDER: S-EPMC10469296 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature