Mammalian display to secretion switchable libraries for antibody preselection and high throughput functional screening.

ABSTRACT: Recently, there has been a co-evolution of mammalian libraries and diverse microfluidic approaches for therapeutic antibody hit discovery. Mammalian libraries enable the preservation of full immune repertoires, produce hit candidates in final format and facilitate broad combinatorial bispecific antibody screening, while several available microfluidic methodologies offer opportunities for rapid high-content screens. Here, we report proof-of-concept studies exploring the potential of combining microfluidic technologies with mammalian libraries for antibody discovery. First, antibody secretion, target co-expression and integration of appropriate reporter cell lines enabled the selection of in-trans acting agonistic bispecific antibodies. Second, a functional screen for internalization

SUBMITTER: Gaa R 

PROVIDER: S-EPMC10469430 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature